Gravity lab bangkok manual#
Gravity lab bangkok full#
Gravity lab bangkok portable#

(C) Photographs of a mass-manufacturable plastic siphonД¬assette that can be operated manually or using a robotic arm, loaded (B) Microfluidic siphon is able to self-prime andДЄllows startвЂ“stop operation with a sequence of reagents withoutД®mptying insets show key steps for a sandwich ELISA with optical/fluorescence The fluid goes throughДЄ neck at liquid high above the inlet before being discharged intoДЄ waste well/tray. With the tip of the siphon with a hydrostatic liquid head, H (driving force for operation). Shown in different colors) are automatically aspirated upon contact Liquid sample, reagents, and washing buffer (labeled R1вЂ“R6, Operation of microfluidic siphons for multistep bioassays.

Gravity lab bangkok portable#

Diagnostic siphons provide the opportunity for high-performance immunoassay testing outside sophisticated laboratories, meeting the rapidly changing global clinical and public health needs.Д«iosensor dengue NS1 immunoassays microfluidic porous membrane portable ELISA siphon smartphone diagnostics. We demonstrated applications of a 10-bore microfluidic siphon as a portable ELISA system without compromised quantitative capabilities in two global diagnostic applications: (1) a four-plex sandwich ELISA for rapid smartphone dengue serotype identification by serotype-specific dengue virus NS1 antigen detection, relevant for acute dengue fever diagnosis, and (2) quantitation of anti-SARS-CoV-2 IgG and IgM titers in spiked serum samples.

Gravity lab bangkok full#

In contrast to other POC devices proposed to date, the operation of the siphon is only dependent on the hydrostatic liquid pressure (gravity) and not capillary forces, and the unique stepwise approach to the delivery of the sample and immunoassay reagents results in zero dead volume in the device, no reagent overlap or carryover, and full start/stop fluid control. We demonstrated that gravity-driven siphon flow can be realized in single-bore glass capillaries, a multibored microcapillary film, and a glass fiber porous membrane. Here we introduce a microfluidic siphon concept that conceptualizes a multistep вЂіdipstickвЂі for quantitative, enzymatically amplified immunoassays using a strip of microporous or microbored material.

Gravity lab bangkok manual#

High analytical performance is intrinsically linked to the use of a sequence of reagent and washing steps, yet this is extremely challenging to deliver at the POC without a high level of fluidic control involving, e.g., automation, fluidic pumping, or manual fluid handling/pipetting. 6 Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.ДЄ range of biosensing techniques including immunoassays are routinely used for quantitation of analytes in biological samples and available in a range of formats, from centralized lab testing (e.g., microplate enzyme-linked immunosorbent assay (ELISA)) to automated point-of-care (POC) and lateral flow immunochromatographic tests.

5 Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 73170, Thailand.

4 Dengue Hemorrhagic Fever Research Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.

3 School of Biological Sciences, University of Reading, Whiteknights Campus, Reading, RG6 6AJ, United Kingdom.

2 Reading School of Pharmacy, University of Reading, Whiteknights Campus, Reading, RG6 6AD United Kingdom.

1 Department of Chemical Engineering and Centre for Biosensors, Biodevices and Bioelectronics (C3Bio), University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom.